1AOS

HUMAN ARGININOSUCCINATE LYASE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	287
Detector technology	AREA DETECTOR
Collection date	1994-06
Detector	SIEMENS
Spacegroup name	P 31 2 1
Unit cell lengths	104.600, 104.600, 183.200
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 4.200
R-factor	0.1879
Rwork	0.188
R-free	0.29800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	22.460 *
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000
High resolution limit [Å]	4.000
Rmerge	0.116 *
Total number of observations	23049 *
Number of reflections	8659 *
Completeness [%]	92.3
Redundancy	2.66

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.1		PROTEIN CRYSTALLIZED FROM 1.1M PHOSPHATE, pH 7.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	drop	phosphate	50 (mM)
3	1	drop	dithiothreitol	5 (mM)
4	1	reservoir	phosphate	1.1 (M)