1AOQ

CYTOCHROME CD1 NITRITE REDUCTASE WITH SUBSTRATE AND PRODUCT BOUND

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID09
Synchrotron site	ESRF
Beamline	ID09
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-04-01
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	106.700, 60.600, 100.100
Unit cell angles	90.00, 112.30, 90.00

Refinement procedure

Resolution	20.000 * - 1.800
R-factor	0.176
Rwork	0.176
R-free	0.19700
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.029 *
Total number of observations	303438 *
Number of reflections	101329
Completeness [%]	94.4	88.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		7		2.3 M AMMONIUM SULFATE 50MM POTASSIUM PHOSPHATE PH 7.0 CRYSTALS WERE REDUCED USING 16MM SODIUM DITHIONITE. CRYSTAL 2 SOAKED IN NITRITE.