1AOI
COMPLEX BETWEEN NUCLEOSOME CORE PARTICLE (H3,H4,H2A,H2B) AND 146 BP LONG DNA FRAGMENT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-08-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 106.040, 181.780, 110.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.800 |
| R-factor | 0.224 * |
| Rwork | 0.224 |
| R-free | 0.30200 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.300 |
| Data reduction software | IN-HOUSE (SOFTWARE) |
| Data scaling software | IN-HOUSE (SOFTWARE) |
| Phasing software | IN-HOUSE (SOFTWARE) |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.850 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.056 | 0.132 |
| Total number of observations | 297397 * | |
| Number of reflections | 52487 | |
| Completeness [%] | 99.0 | 95 |
| Redundancy | 5.7 | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 4 (mg/ml) | |
| 2 | 1 | drop | 50 (mM) | ||
| 3 | 1 | drop | 70-75 (mM) | ||
| 4 | 1 | drop | potassium cacodylate | 20 (mM) | pH6.0 |
| 5 | 1 | reservoir | 40-46 (mM) | ||
| 6 | 1 | reservoir | 35-40 (mM) | ||
| 7 | 1 | reservoir | potassium cacodylate | 20 (mM) | pH6.0 |
