1AOH
SINGLE COHESIN DOMAIN FROM THE SCAFFOLDING PROTEIN CIPA OF THE CLOSTRIDIUM THERMOCELLUM CELLULOSOME
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | LURE BEAMLINE DW32 |
| Synchrotron site | LURE |
| Beamline | DW32 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.840, 80.500, 93.240 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.26000 * |
| Structure solution method | MIR |
| RMSD bond length | 0.014 |
| RMSD bond angle | 28.240 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.055 | 0.253 |
| Total number of observations | 175259 * | |
| Number of reflections | 29669 | |
| <I/σ(I)> | 22.2 | 4.5 |
| Completeness [%] | 92.2 | 86.6 |
| Redundancy | 5.8 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.25 | Beguin, P., (1996) Protein Sci., 5, 1192 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 18 (%(w/v)) | |
| 2 | 1 | reservoir | calcium acetate | 0.2 (M) | |
| 3 | 1 | reservoir | glycerol | 6 (%(v/v)) | |
| 4 | 1 | reservoir | sodium cacodylate | 0.05 (M) | |
| 5 | 1 | drop | PEG8000 | 9 (%(w/v)) | |
| 6 | 1 | drop | calcium acetate | 0.1 (M) | |
| 7 | 1 | drop | glycerol | 3 (%(v/v)) | |
| 8 | 1 | drop | sodium cacodylate | 0.025 (M) | pH6.25 |
| 9 | 1 | drop | protein | 7.5-10 (mg/ml) |
