1AOF
CYTOCHROME CD1 NITRITE REDUCTASE, REDUCED FORM
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.5 |
| Synchrotron site | SRS |
| Beamline | PX9.5 |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-03-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.400, 61.700, 101.200 |
| Unit cell angles | 90.00, 112.10, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.17 * |
| Rwork | 0.170 |
| R-free | 0.20100 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.984 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.8) |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.300 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.062 * | |
| Total number of observations | 461872 * | |
| Number of reflections | 58965 | |
| Completeness [%] | 93.5 | 91.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 15 * | Fulop, V., (1993) J. Mol. Biol., 232, 1211. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 1.1-1.15 (M) | |
| 3 | 1 | drop | potassium phosphate | 25 (mM) | |
| 4 | 1 | reservoir | ammonium sulfate | 2.2-2.3 (M) | |
| 5 | 1 | reservoir | potassium phosphate | 50 (mM) |
