1AOD

PHOSPHATIDYLINOSITOL-SPECIFIC PHOSPHOLIPASE C FROM LISTERIA MONOCYTOGENES

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1996-10-28
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	82.100, 82.100, 92.100
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.158
Rwork	0.158
R-free	0.21900 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ptd
RMSD bond length	0.006
RMSD bond angle	23.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000	2.640
High resolution limit [Å]	2.600	2.600
Rmerge	0.104 *	0.380
Total number of observations	43095 *
Number of reflections	10914
<I/σ(I)>	7.7	3.2
Completeness [%]	95.3 *	97
Redundancy	3.9	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	4 *	THE PROTEIN WAS CRYSTALLIZED FROM 0.1 M NA-HEPES PH 7.5, 0.8 M NA-K-TARTRATE, 45 MM MYO-INOSITOL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	6.5 (mg/ml)
2	1	drop	precipitant
3	1	reservoir	Na-Hepes	0.1 (M)
4	1	reservoir	Na/K-tartrate	0.8 (M)