1AO0
GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE (PRPP) AMIDOTRANSFERASE FROM B. SUBTILIS COMPLEXED WITH ADP AND GMP
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 160.300, 70.400, 182.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.214 |
| Rwork | 0.214 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gph |
| RMSD bond length | 0.016 |
| RMSD bond angle | 2.390 * |
| Data reduction software | HKL ((DENZO)) |
| Data scaling software | HKL |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 15.000 |
| High resolution limit [Å] | 2.800 |
| Rmerge | 0.080 * |
| Total number of observations | 195956 * |
| Number of reflections | 46771 |
| <I/σ(I)> | 20.2 |
| Completeness [%] | 91.0 |
| Redundancy | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7.9 | 24% PEG8000, 200MM KCL, 50MM EPPS PH 7.9,2 MM ADP, 2 MM GMP, 10 MM MGCL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 10 | 1 | 2 | 5 (mM) | ||
| 2 | 1 | 1 | ADP | 1 (mM) | |
| 3 | 1 | 1 | GMP | 1 (mM) | |
| 4 | 1 | 1 | 5 (mM) | ||
| 5 | 1 | 2 | PEG8000 | 24 (%) | |
| 6 | 1 | 2 | 200 (mM) | ||
| 7 | 1 | 2 | EPPS | 50 (mM) | |
| 8 | 1 | 2 | ADP | 1 (mM) | |
| 9 | 1 | 2 | GMP | 1 (mM) |
