1ANV

ADENOVIRUS 5 DBP/URANYL FLUORIDE SOAK

Experimental procedure

Source type	ROTATING ANODE
Source details	MACSCIENCE M18X
Temperature [K]	295
Detector technology	IMAGE PLATE
Collection date	1994-05-27
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	79.700, 75.600, 60.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 2.700
R-factor	0.206 *
Rwork	0.204
R-free	0.31400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ADT
RMSD bond length	0.010
RMSD bond angle	26.800 *
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	TNT

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	3.200
High resolution limit [Å]	2.700	2.700
Rmerge	0.147	0.342
Total number of observations	34497 *
Number of reflections	10312
Completeness [%]	98.7	99.7
Redundancy	3.4	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.8		Tsernoglou, D., (1984) J. Mol. Biol., 172, 237. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.5 (%)
2	1	drop	ammonium sulfate	13 (%sat)