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THE CLOSED CONFORMATION OF A HIGHLY FLEXIBLE PROTEIN: THE STRUCTURE OF E. COLI ADENYLATE KINASE WITH BOUND AMP AND AMPPNP

Experimental procedure

Spacegroup name	C 1 2 1
Unit cell lengths	109.700, 73.500, 79.600
Unit cell angles	90.00, 131.97, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.201
Rwork	0.201
R-free	0.31600
RMSD bond length	0.019
RMSD bond angle	2.350
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	15.000 *
High resolution limit [Å]	2.000 *
Total number of observations	73378 *
Number of reflections	30315 *
Completeness [%]	85.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2.3 (M)
2	1	reservoir	imidazole	100 (mM)
3	1	reservoir	AMP	10 (mM)
4	1	reservoir	AMPPNP	10 (mM)
5	1	reservoir		2 (mM)
6	1	reservoir	protein	25 (mg/ml)