1ANJ
ALKALINE PHOSPHATASE (K328H)
Experimental procedure
| Detector technology | AREA DETECTOR |
| Collection date | 1994-07-27 |
| Detector | SAN DIEGO MULTIWIRE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 195.100, 167.300, 76.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.300 |
| R-factor | 0.194 |
| Rwork | 0.194 |
| R-free | 0.24900 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 24.200 * |
| Data reduction software | SDMS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.080 |
| Total number of observations | 214198 * |
| Number of reflections | 55520 |
| Completeness [%] | 99.0 |
| Redundancy | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 9.5 * | pH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 30 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 20 (%sat) | |
| 3 | 1 | drop | Tris-HCl | 100 (mM) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | drop | 0.01 (mM) | ||
| 6 | 1 | reservoir | ammonium sulfate | 36-39 (%sat) |
