1AN2
RECOGNITION BY MAX OF ITS COGNATE DNA THROUGH A DIMERIC B/HLH/Z DOMAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 258 |
| Detector technology | IMAGE PLATE |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 72.200, 72.200, 146.400 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.900 |
| R-factor | 0.232 |
| Rwork | 0.232 |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 2.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.065 * | 0.260 * |
| Number of reflections | 4507 | |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 85.3 | 61 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 4 * | pH 5.50, VAPOR DIFFUSION, SITTING DROP, temperature 277.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 10 | 1 | 2 | KCL | ||
| 11 | 1 | 2 | MGCL2 | ||
| 12 | 1 | 2 | NA CACODYLATE | ||
| 2 | 1 | 1 | PEG 1000 | ||
| 3 | 1 | 1 | GLYCEROL | ||
| 4 | 1 | 1 | KCL | ||
| 5 | 1 | 1 | MGCL2 | ||
| 6 | 1 | 1 | NA CACODYLATE | ||
| 7 | 1 | 2 | WATER | ||
| 8 | 1 | 2 | PEG 1000 | ||
| 9 | 1 | 2 | GLYCEROL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG1000 | 4.5-7.5 (%) | |
| 2 | 1 | reservoir | glycerol | 5-10 (%) | |
| 3 | 1 | reservoir | 100 (mM) | ||
| 4 | 1 | reservoir | 2 (mM) | ||
| 5 | 1 | reservoir | sodium cacodylate | 100 (mM) |
