1AMY
CRYSTAL AND MOLECULAR STRUCTURE OF BARLEY ALPHA-AMYLASE
Experimental procedure
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 135.200, 135.200, 79.600 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.800 |
| R-factor | 0.153 |
| Rwork | 0.153 |
| RMSD bond length | 0.016 |
| RMSD bond angle | 3.300 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 2.770 * |
| Rmerge | 0.151 * |
| Total number of observations | 109747 * |
| Number of reflections | 19542 * |
| Completeness [%] | 89.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.7 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5-10 (mg/ml) | |
| 2 | 1 | drop | Mes | 10 (mM) | |
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | 5 (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | 0.4-1.0 (M) |
