1AMM

1.2 ANGSTROM STRUCTURE OF GAMMA-B CRYSTALLIN AT 150K

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	150
Detector technology	FILM
Collection date	1992-08
Detector	CEA FILM
Spacegroup name	P 41 21 2
Unit cell lengths	56.430, 56.430, 97.120
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.000 - 1.200
R-factor	0.185 *
RMSD bond length	0.019
Data reduction software	MOSFLM
Refinement software	RESTRAIN

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.260
High resolution limit [Å]	1.180	1.200
Rmerge	0.077	0.200
Total number of observations	173126 *
Number of reflections	45977	5317 *
<I/σ(I)>	6.8	3.3
Completeness [%]	94.0	88
Redundancy	3.76	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *		0 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	70 (mg/ml)
2	1	1	sodium hydrogen phosphate	0.05 (M)
3	1	1	dithiothreitol	1 (mM)