1AMH
UNCOMPLEXED RAT TRYPSIN MUTANT WITH ASP 189 REPLACED WITH SER (D189S)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-12 |
| Detector | RIGAKU |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.920, 49.790, 89.880 |
| Unit cell angles | 90.00, 97.34, 90.00 |
Refinement procedure
| Resolution | 100.000 - 2.500 |
| R-factor | 0.182 |
| Rwork | 0.182 |
| R-free | 0.24200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1brb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 26.170 * |
| Data reduction software | bioteX |
| Data scaling software | bioteX |
| Phasing software | X-PLOR (3.851) |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.600 |
| High resolution limit [Å] | 2.520 | 2.540 * |
| Rmerge | 0.120 | 0.296 |
| Number of reflections | 13312 | 470 * |
| <I/σ(I)> | 7.7 | 2.63 |
| Completeness [%] | 94.5 | 91.9 |
| Redundancy | 3.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 | 4 * | CRYSTALLIZED AT 277 K FROM 10 MG/ML PROTEIN SOLUTION USING 22% PEG 3350, 10 MM CACL2 AND 0.15M NH4CL AS PRECIPITANT AT PH=5.6 (0.1 M SODIUM CITRATE). |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | sodium citrate | 0.1 (M) | |
| 3 | 1 | reservoir | 10 (mM) | ||
| 4 | 1 | reservoir | PEG3350 | 22 (%) | |
| 5 | 1 | reservoir | ammonium chloride | 0.15 (M) |
