1AM7

Lysozyme from bacteriophage lambda

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-05-03
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	73.010, 78.800, 82.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	9999.000 * - 2.300
R-factor	0.1635 *
Rwork	0.163
R-free	0.21300
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	21.100 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.090 *	0.297 *
Total number of observations	104257 *
Number of reflections	21455
<I/σ(I)>	15	3.4
Completeness [%]	98.8	93.5
Redundancy	4.9	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	5.3		protein solution is mixed 50:50 with a well solution *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop		50 (mM)
3	1	drop		0.02 (%(w/v))
4	1	reservoir	PEG4000	20 (%(w/v))
5	1	reservoir	2-propanol	15 (%(v/v))
6	1	reservoir	sodium citrate	0.1 (M)