1AM2
GYRA INTEIN FROM MYCOBACTERIUM XENOPI
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-03-23 |
| Detector | MACSCIENCE |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 55.700, 55.700, 133.110 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 2.200 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.25200 |
| Structure solution method | MULTIPLE ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.462 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.049 | 0.250 |
| Total number of observations | 58763 * | |
| Number of reflections | 14058 | |
| <I/σ(I)> | 15.2 | 4.65 |
| Completeness [%] | 96.1 | 96.8 |
| Redundancy | 4.18 | 3.67 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.6 * | pH 5.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | (mg/ml) | |
| 2 | 1 | drop | dithiothreitol | (mM) | |
| 3 | 1 | reservoir | sodium citrate | (mM) | |
| 4 | 1 | reservoir | (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | (mM) |
