Experimental procedure
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 120 |
| Detector technology | AREA DETECTOR |
| Collection date | 1987-01 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 31.595, 32.369, 24.219 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 100.000 - 0.860 |
| R-factor | 0.162 |
| R-free | 0.18500 |
| Structure solution method | DIRECT METHODS, FOURIER RECYCLING |
| RMSD bond length | 0.016 |
| RMSD bond angle | 0.032 |
| Data reduction software | SIEMENS |
| Data scaling software | SIEMENS |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 0.900 |
| High resolution limit [Å] | 0.860 | 0.860 |
| Rmerge | 0.164 * | 0.043 |
| Number of reflections | 21454 | |
| <I/σ(I)> | 5.1 | 1.2 |
| Completeness [%] | 99.9 | 93.6 |
| Redundancy | 1.1 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7 | CRYSTALLIZED BY BATCH METHODS FROM A SATURATED SOLUTION OF GRAMICIDIN D IN ETHANOL., PH 7.0, BATCH METHOD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 30 (mg/ml) | |
| 2 | 1 | 1 | PEG4000 | 2 (%(w/v)) | |
| 3 | 1 | 1 | n-propanol |
