1ALY
CRYSTAL STRUCTURE OF HUMAN CD40 LIGAND
Experimental procedure
| Temperature [K] | 123 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-01-11 |
| Detector | SIEMENS |
| Spacegroup name | H 3 |
| Unit cell lengths | 77.170, 77.170, 90.460 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 7.500 - 2.000 |
| R-factor | 0.223 |
| Rwork | 0.223 |
| R-free | 0.29500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1CDA |
| RMSD bond length | 0.021 * |
| RMSD bond angle | 3.900 * |
| Data reduction software | BUDDHA |
| Data scaling software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.067 | 0.056 |
| Total number of observations | 42587 * | |
| Number of reflections | 15693 * | |
| <I/σ(I)> | 6.5 | 3.9 |
| Completeness [%] | 77.1 | 46.1 |
| Redundancy | 2.71 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | CD40L | 0.5 (mM) | |
| 3 | 1 | drop | 0.144 (M) | ||
| 4 | 1 | drop | 0.056 (M) | ||
| 5 | 1 | drop | 1.5 (M) | ||
| 6 | 1 | reservoir | Na-citrate | 1.4 (M) | |
| 7 | 1 | reservoir | Na-HEPES | 50 (mM) |
