1ALV
CALCIUM BOUND DOMAIN VI OF PORCINE CALPAIN
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 280 |
| Detector technology | AREA DETECTOR |
| Collection date | 1996-08 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.870, 80.460, 57.080 |
| Unit cell angles | 90.00, 91.20, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.23000 |
| Structure solution method | MIR |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.422 |
| Data reduction software | X-GEN |
| Data scaling software | X-GEN |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.050 |
| Number of reflections | 33812 | |
| <I/σ(I)> | 15 | 3.5 |
| Completeness [%] | 97.0 | 90 |
| Redundancy | 3 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | PROTEIN WAS CRYSTALLIZED FROM 12% PEG6000 20 MM CACL2, 50 MM CACODYLATE BUFFER, PH7.4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG6000 |
