1ALK

REACTION MECHANISM OF ALKALINE PHOSPHATASE BASED ON CRYSTAL STRUCTURES. TWO METAL ION CATALYSIS

Experimental procedure

Spacegroup name	I 2 2 2
Unit cell lengths	195.300, 167.400, 76.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	5.000 * - 2.000
R-factor	0.177
Rwork	0.177
RMSD bond length	0.015 *
RMSD bond angle	2.960 *
Phasing software	X-PLOR
Refinement software	PROFFT

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.047 *
Number of reflections	17164 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	9.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	0.1 (M)
2	1	1		0.01 (M)
3	1	1		5x10-5 (M)