1ALJ

ALKALINE PHOSPHATASE MUTANT (H412N)

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993-05-14
Detector	ADSC
Spacegroup name	I 2 2 2
Unit cell lengths	194.760, 167.740, 76.230
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.600
R-factor	0.166
Rwork	0.166
RMSD bond length	0.020
RMSD bond angle	22.800 *
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	26.300
High resolution limit [Å]	2.500
Rmerge	0.108
Number of reflections	36882
Completeness [%]	98.4
Redundancy	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9.5 *		THIS ENTRY IS A MUTANT ALKALINE PHOSPHATE (H412N) IN WHICH HIS 412 IS REPLACED BY ASN, DETERMINED WITH CRYSTALS SOAKED IN STABILIZATION BUFFER CONTAINING NO ADDED ZINC. THERE IS ONE ZINC AND ONE MAGNESIUM COMPLEXED WITH INORGANIC PHOSPHATE BOUND IN EACH OF THE TWO ACTIVE SITES.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	39 (%sat)
2	1	drop	Tris-HCl	100 (mM)
3	1	drop		10 (mM)