1ALI

ALKALINE PHOSPHATASE MUTANT (H412N)

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1994-01-23
Detector	ADSC
Spacegroup name	I 2 2 2
Unit cell lengths	194.860, 167.430, 76.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.174
Rwork	0.174
RMSD bond length	0.020
RMSD bond angle	23.000 *
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	39.300
High resolution limit [Å]	2.100
Rmerge	0.087
Total number of observations	224987 *
Number of reflections	63998
Completeness [%]	99.3
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9.5 *		THE STRUCTURE IS OF A MUTANT ALKALINE PHOSPHATE (H412N) IN WHICH HIS 412 IS REPLACED BY ASN, DETERMINED WITH CRYSTALS SOAKED IN STABILIZATION BUFFER CONTAINING 10 MM ZINC CHLORIDE. THERE ARE TWO ZINCS AND ONE MAGNESIUM COMPLEXED WITH AN INORGANIC PHOSPHATE BOUND IN EACH OF THE TWO ACTIVE SITES.

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	39 (%sat)
2	1	drop	Tris-HCl	100 (mM)
3	1	drop		10 (mM)