1AL7
THREE-DIMENSIONAL STRUCTURES OF GLYCOLATE OXIDASE WITH BOUND ACTIVE-SITE INHIBITORS
Experimental procedure
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-10 |
| Detector | RIGAKU RAXIS II |
| Spacegroup name | I 4 2 2 |
| Unit cell lengths | 148.100, 148.100, 136.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.600 |
| R-factor | 0.18 |
| Rwork | 0.180 |
| R-free | 0.24200 |
| Structure solution method | DIFFERENCE FOURIER |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.952 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 2.680 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.085 | 0.206 |
| Total number of observations | 48698 * | |
| Number of reflections | 20291 | |
| <I/σ(I)> | 6.99 | 3.1 |
| Completeness [%] | 87.1 | 74.4 |
| Redundancy | 2.4 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 8.3 | PROTEIN WAS CRYSTALLISED FROM 50MM TRIS-BUFFER PH 8.3, 0.25 MG/ML FMN, 4% TERTIARY BUTANOL, SATURATED WITH THE INHIBITOR. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 20 (mg/ml) | |
| 2 | 1 | 1 | Tris-HCl | 0.05 (M) | |
| 3 | 1 | 1 | GOX | 0.5 (mg/ml) | |
| 4 | 1 | 1 | FMN | 0.25 (mg/ml) | |
| 5 | 1 | 1 | JF5969 | 0.5 (%) | |
| 6 | 1 | 2 | tertiary butanol | 5 (%(v/v)) |
