1AKT

G61N OXIDIZED FLAVODOXIN MUTANT

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-08
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	56.140, 87.580, 34.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.800
R-factor	0.181 *
Rwork	0.183
R-free	0.21300 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010 *
RMSD bond angle	0.041
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	AMoRE
Refinement software	PROLSQ

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000
High resolution limit [Å]	1.800
Rmerge	0.026 *	0.278 *
Total number of observations	50204 *
Number of reflections	14539
<I/σ(I)>	19
Completeness [%]	88.0	86.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7	18 *	60-70% AMMONIUM SULFATE, 100MM TRIS-HCL, pH 7.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	acetone	1-2 (%(v/v))
2	1	drop	Tris-HCl	10 (mM)
3	1	drop	EDTA	1 (mM)
4	1	drop	ammonium sulfate	60-70 (%)