1AKS

CRYSTAL STRUCTURE OF THE FIRST ACTIVE AUTOLYSATE FORM OF THE PORCINE ALPHA TRYPSIN

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	77.700, 53.820, 47.080
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.800
R-factor	0.2
Rwork	0.200
R-free	0.28500 *
RMSD bond length	0.011
RMSD bond angle	26.850 *
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	1.800 *	1.800 *
Rmerge	0.080 *	0.661 *
Total number of observations	56502 *
Number of reflections	17486 *	4585 *
Completeness [%]	95.0 *	91.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.7 *	293 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop		20 (mM)
3	1	drop	sodium acetate	0.05 (M)
4	1	reservoir	ammonium sulfate	1.5 (M)
5	1	drop	ammonium sulfate	0.04 (M)