1AKH
MAT A1/ALPHA2/DNA TERNARY COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 94 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05 |
| Detector | RIGAKU |
| Spacegroup name | P 61 |
| Unit cell lengths | 132.250, 132.250, 45.250 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.201 * |
| Rwork | 0.201 |
| R-free | 0.30200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yrn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.870 |
| Data reduction software | R-AXIS |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.480 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.066 * | |
| Total number of observations | 31013 * | |
| Number of reflections | 15656 | |
| Completeness [%] | 83.6 | 67 |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 * | 295 | 100MM HEPES PH 7.0, 20MM CACL2, 5MM [CO(NH3)6]CL3., VAPOR DIFFUSION, HANGING DROP, temperature 295.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | HEPES BUFFER | ||
| 3 | 1 | 1 | CACL2 | ||
| 4 | 1 | 1 | COBALT HEXAMINE | ||
| 5 | 1 | 2 | WATER | ||
| 6 | 1 | 2 | HEPES BUFFER | ||
| 7 | 1 | 2 | CACL2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 50 (mM) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | cobaltic hexamine | 2.5-5 (mM) | |
| 4 | 1 | reservoir | HEPES | 150-200 (mM) | |
| 5 | 1 | reservoir | 20 (mM) |
