1AK1
FERROCHELATASE FROM BACILLUS SUBTILIS
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 287 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-10-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.390, 50.190, 120.120 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.195 |
| Rwork | 0.195 |
| R-free | 0.24300 |
| Structure solution method | MIR |
| RMSD bond length | 0.008 |
| RMSD bond angle | 23.250 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.063 * | 0.364 * |
| Total number of observations | 226242 * | |
| Number of reflections | 24332 | |
| Completeness [%] | 92.4 | 80.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 | CRYSTALS GROW IN 30% PEG 2000, 0.2 M MGCL2 0.1 M TRIS-HCL, PH 8.5. THESE WERE VORTEXED AND USED AS SEEDS FOR LARGE SINGLE CRYSTALS IN: 27% PEG 2000, 0.2 M MGCL2, 0.05% NAN3, 0.1 M TRIS-HCL, PH 8.5, seeded |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.65 (mg/ml) | |
| 2 | 1 | drop | PEG2000 | 2.5 (%) | |
| 3 | 1 | drop | 17 (mM) | ||
| 4 | 1 | drop | Tris-HCl | 8 (mM) | |
| 5 | 1 | reservoir | PEG2000 | 30 (%(w/v)) | |
| 6 | 1 | reservoir | 0.2 (M) | ||
| 7 | 1 | reservoir | Tris-HCl | 0.1 (M) |
