1AJK
CIRCULARLY PERMUTED (1-3,1-4)-BETA-D-GLUCAN 4-GLUCANOHYDROLASE CPA16M-84
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 294 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.450, 65.820, 69.610 |
| Unit cell angles | 90.00, 106.87, 90.00 |
Refinement procedure
| Resolution | 33.330 - 1.800 |
| Rwork | 0.168 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ayh |
| RMSD bond length | 0.015 |
| RMSD bond angle | 0.033 |
| Data reduction software | DENZO |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.330 | 1.830 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Rmerge | 0.056 | 0.164 |
| Total number of observations | 132154 * | |
| Number of reflections | 38698 | |
| <I/σ(I)> | 5.8 | 4.5 |
| Completeness [%] | 98.3 | 94.4 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | SITTING DROP METHOD: A SOLUTION OF 12 MG OF PROTEIN PER ML IN 10 MM HEPES, PH 7.0, 2 MM CA-CHLORIDE, MIXED WITH AN EQUAL VOLUME OF 50 MM K-PHOSPHATE, PH 7.0 AND 20% (BY WEIGHT) PEG 8000, vapor diffusion - sitting drop |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6 (mg/ml) | |
| 2 | 1 | drop | HEPES | 5 (mM) | |
| 3 | 1 | drop | 1 (mM) | ||
| 4 | 1 | drop | potassium phosphate | 25 (mM) | |
| 5 | 1 | drop | PEG8000 | 10 (%) | |
| 6 | 1 | reservoir | potassium phosphate | 50 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 20 (%) |
