1AJA

THREE-DIMENSIONAL STRUCTURE OF THE D153G MUTANT OF E. COLI ALKALINE PHOSPHATASE: A MUTANT WITH WEAKER MAGNESIUM BINDING AND INCREASED CATALYTIC ACTIVITY

Experimental procedure

Detector technology	IMAGE PLATE
Collection date	1994-01-06
Detector	RIGAKU RAXIS
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	195.020, 166.930, 76.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.162
Rwork	0.162
RMSD bond length	0.013 *
RMSD bond angle	2.200 *
Data reduction software	R-AXIS
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.080
Number of reflections	65477 *
Completeness [%]	76.5 *
Redundancy	1.65

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	9.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	30 (mg/ml)
2	1	drop	ammonium sulfate	20 (%(w/v))
3	1	drop	Tris-HCl	100 (mM)
4	1	drop		10 (mM)
5	1	drop		0.01 (mM)
6	1	reservoir	ammonium sulfate	31.6 (%)
7	1	reservoir	Tris-HCl	100 (mM)
8	1	reservoir		10 (mM)