1AJ2
CRYSTAL STRUCTURE OF A BINARY COMPLEX OF E. COLI DIHYDROPTEROATE SYNTHASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 289 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-05 |
| Detector | SIEMENS |
| Spacegroup name | C 1 21 1 |
| Unit cell lengths | 93.500, 60.940, 59.930 |
| Unit cell angles | 90.00, 114.80, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.169 |
| Rwork | 0.169 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 3.180 |
| Data reduction software | XENGEN |
| Data scaling software | XENGEN |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.200 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.067 * | |
| Total number of observations | 23600 * | |
| Number of reflections | 14242 | |
| Completeness [%] | 69.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 8.4 | PROTEIN WAS CRYSTALLIZED FROM 42% AMMONIUM SULFATE, 50MM TRIS-HCL, PH 8.4. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | ammonium sulfate | 42 (%sat) | |
| 2 | 1 | 1 | Tris-HCl | 50 (mM) |
