1AIS
TATA-BINDING PROTEIN/TRANSCRIPTION FACTOR (II)B/TATA-BOX COMPLEX FROM PYROCOCCUS WOESEI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-01 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.700, 91.200, 74.200 |
| Unit cell angles | 90.00, 122.70, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.212 |
| Rwork | 0.212 |
| R-free | 0.26800 |
| Structure solution method | MIR |
| RMSD bond length | 0.009 |
| RMSD bond angle | 22.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 14445 | |
| <I/σ(I)> | 3.7 | |
| Completeness [%] | 92.0 | 96.2 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 18 * | HANGING DROP METHOD 8% PEG 8000, 200 MM POTASSIUM PHOSPHATE PH 7.4., vapor diffusion - hanging drop |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | K PHOSPHATE | ||
| 3 | 1 | 1 | PEG 8000 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | PEG8000 | 4 (%(w/v)) | |
| 2 | 1 | drop | potassium phosphate | 100 (mM) | |
| 3 | 1 | drop | DNA complex | 0.15 (mM) | |
| 4 | 1 | drop | potassium acetate | 75 (mM) | |
| 5 | 1 | drop | Tris | 5 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 8 (%) | |
| 7 | 1 | reservoir | potassium phosphate | 200 (mM) |
