1AIN

CRYSTAL STRUCTURE OF HUMAN ANNEXIN I AT 2.5 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	139.360, 67.500, 42.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.500
R-factor	0.177 *
Rwork	0.163
RMSD bond length	0.014 *
RMSD bond angle	2.700 *
Phasing software	X-PLOR
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.053 *
Total number of observations	76668 *
Number of reflections	13945 *
Completeness [%]	97.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	6.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein solution	20 (mg/mL)
2	1	drop	cacodylate	0.05 (M)
3	1	drop	Na(OAc)	0.1 (M)
4	1	drop		10 (mM)
5	1	drop	PEG4000	8 (%)
6	1	reservoir	cacodylate	0.1 (M)
7	1	reservoir	Na(OAc)	0.2 (M)
8	1	reservoir		10 (mM)
9	1	reservoir	PEG4000	16 (%)