1AIG
PHOTOSYNTHETIC REACTION CENTER FROM RHODOBACTER SPHAEROIDES IN THE D+QB-CHARGE SEPARATED STATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 77 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 140.100, 140.100, 271.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.215 |
| Rwork | 0.215 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 22.200 * |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | MERLOT |
| Refinement software | X-PLOR (3.89) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.720 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.085 * | 0.160 * |
| Total number of observations | 203621 * | |
| Number of reflections | 74104 | |
| <I/σ(I)> | 14.3 | 2.6 |
| Completeness [%] | 88.0 | 86 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8 * | Allen, J.P., (1994) Proteins. Struct. Funct. Genet., 20, 283. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-Cl | 10 (mM) | |
| 3 | 1 | drop | beta-octylglucoside | 0.85 (%) | |
| 4 | 1 | drop | PEG4000 | 6.0 (%) | |
| 5 | 1 | drop | amphiphiles benzamidine hydrochloride | 0.4 (%) | |
| 6 | 1 | drop | heptane triol | 2.5 (%) | |
| 7 | 1 | reservoir | PEG4000 | 32 (%) |
