1AIE

P53 TETRAMERIZATION DOMAIN CRYSTAL STRUCTURE

Experimental procedure

Source type	SYNCHROTRON
Source details	ESRF
Synchrotron site	ESRF
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1995-10-20
Detector	MARRESEARCH
Spacegroup name	P 4 2 2
Unit cell lengths	45.500, 45.500, 33.200
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.500
R-factor	0.191
Rwork	0.191
R-free	0.25200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1pet
RMSD bond length	0.010
RMSD bond angle	22.990 *
Data reduction software	XDS
Data scaling software	MARSCALE
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	26.900	1.600
High resolution limit [Å]	1.500	1.500
Rmerge	0.069 *	0.421 *
Number of reflections	5276	852 *
<I/σ(I)>	25.31	4.3
Completeness [%]	94.6	90.7
Redundancy	5	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8		1.4 M SODIUM CITRATE, 100 MM HEPES, PH 8.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	1.4 (M)
2	1	reservoir	HEPES	100 (mM)