1AIB

STRUCTURAL BASIS FOR THE CATALYTIC ACTIVITY OF ASPARTATE AMINOTRANSFERASE K258H LACKING THE PYRIDOXAL-5'-PHOSPHATE BINDING LYSINE RESIDUE

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	86.600, 79.800, 89.800
Unit cell angles	90.00, 119.00, 90.00

Refinement procedure

Resolution	10.000 - 2.800
R-factor	0.219
Rwork	0.219
RMSD bond length	0.015
RMSD bond angle	3.190
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	2.800 *
Rmerge	0.091 *
Number of reflections	27007 *
Completeness [%]	98.4 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15.1 (mg/mL)
2	1	drop	maleate	100 (mM)
3	1	drop	sodium chloride	0.45-0.55 (M)
4	1	drop	PEG	16 (%(w/v))
5	1	drop	sodium phosphate	20 (mM)
6	1	reservoir	PEG	32 (%(w/v))
7	1	reservoir	sodium chloride	0.9-1.1 (M)
8	1	reservoir	sodium phosphate	20 (mM)