1AHQ

RECOMBINANT ACTOPHORIN

Experimental procedure

Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	1995-05-12
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 21 21 21
Unit cell lengths	39.295, 47.486, 67.585
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.300
R-factor	0.21
Rwork	0.210
R-free	0.32000
Structure solution method	MIR X-RAY CRYSTAL
RMSD bond length	0.016
RMSD bond angle	1.340 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.250
High resolution limit [Å]	2.060	2.060
Rmerge	0.068 *
Total number of observations	26016 *
Number of reflections	7897
<I/σ(I)>		5.3
Completeness [%]	95.0	92.5
Redundancy	3.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5		20-25% PEG 8000, 100MM HEPES (PH7.5), 10% ISOPROPANOL, 1MM DTT

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)
2	1	reservoir	isopropanol	10 (%)
3	1	reservoir	dithiothreitol	1 (mM)
4	1	reservoir	PEG8000	20-25 (%)