1AH7

PHOSPHOLIPASE C FROM BACILLUS CEREUS

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	300
Detector technology	DIFFRACTOMETER
Collection date	1986-08
Detector	ENRAF-NONIUS FAST
Spacegroup name	P 43 21 2
Unit cell lengths	89.760, 89.760, 74.030
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	12.451 - 1.501
R-factor	0.20331
Rwork	0.203
R-free	0.23124
Structure solution method	MULTIPLE ISOMORPHOUS REPLACEMENT
RMSD bond length	0.021 *
RMSD bond angle	0.049 *
Data reduction software	CCP4
Data scaling software	CCP4
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	12.450	1.570
High resolution limit [Å]	1.500	1.501
Rmerge	0.056 *
Number of reflections	46061
Completeness [%]	95.0	82
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7		VAPOR PHASE DIFFUSION FROM SOLUTION OF 5MG/ML PLC IN 35% SATURATED AMMONIUM SULFATE, pH 7, vapor diffusion

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	5 (mg/ml)
2	1	drop	ammonium sulfate	35 (%sat)
3	1	reservoir	ammonium sulfate	2.5 (M)