1AGS

A SURFACE MUTANT (G82R) OF A HUMAN ALPHA-GLUTATHIONE S-TRANSFERASE SHOWS DECREASED THERMAL STABILITY AND A NEW MODE OF MOLECULAR ASSOCIATION IN THE CRYSTAL

Experimental procedure

Detector technology	AREA DETECTOR
Detector	SIEMENS-NICOLET X100
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	49.500, 92.900, 115.900
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.500
R-factor	0.31
Rwork	0.310
Data reduction software	XENGEN (V. 2.1)
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.500 *
Rmerge	0.100
Number of reflections	18098
Completeness [%]	92.6 *
Redundancy	2.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	25 (mM)
3	1	reservoir	PEG3350	18 (%)
4	1	reservoir	EDTA	1 (mM)
5	1	reservoir	1,2,3-heptanetriol	0.05 (%(w/v))
6	1	reservoir	Tris-HCl	100 (mM)