1AGN
X-RAY STRUCTURE OF HUMAN SIGMA ALCOHOL DEHYDROGENASE
Experimental procedure
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05-22 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 86.300, 94.650, 121.660 |
| Unit cell angles | 90.00, 100.03, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.000 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.30500 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 2.000 |
| Data reduction software | R-AXIS (IIC (HAGASHI/RIGAKU)) |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 3.000 * | 3.000 * |
| Rmerge | 0.120 | |
| Total number of observations | 79865 * | |
| Number of reflections | 34754 | |
| Completeness [%] | 87.0 | 83.5 * |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 6.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | reservoir | cacodylate | 100 (mM) | |
| 3 | 1 | reservoir | zinc acetate | 50-100 (mM) | |
| 4 | 1 | reservoir | NAD+ | 7.5 (mM) | |
| 5 | 1 | reservoir | PEG6000 | 18 (%(w/v)) |
