1AG9
FLAVODOXINS THAT ARE REQUIRED FOR ENZYME ACTIVATION: THE STRUCTURE OF OXIDIZED FLAVODOXIN FROM ESCHERICHIA COLI AT 1.8 ANGSTROMS RESOLUTION.
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 140 |
| Detector technology | AREA DETECTOR |
| Collection date | 1994-12 |
| Detector | XUONG-HAMLIN MULTIWIRE |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 126.400, 41.100, 68.150 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ofv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 23.800 * |
| Data reduction software | SDMS |
| Data scaling software | SDMS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.880 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.072 * | 0.166 * |
| Total number of observations | 213264 * | |
| Number of reflections | 32752 | |
| <I/σ(I)> | 26.3 | 5.9 |
| Completeness [%] | 97.1 | 93.4 |
| Redundancy | 6.6 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 22 * | microseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.5-10 (mg/ml) | |
| 2 | 1 | drop | imidazole | 10 (mM) | |
| 3 | 1 | drop | MPD | 20 (%) | |
| 4 | 1 | drop | 50 (mM) | ||
| 5 | 1 | drop | Bis-Tris | 10 (mM) | |
| 6 | 1 | reservoir | MPD | 40 (%) | |
| 7 | 1 | reservoir | 100 (mM) | ||
| 8 | 1 | reservoir | Bis-Tris | 20 (mM) | |
| 9 | 1 | reservoir | imidazole | 10 (mM) |
