1AFW
THE 1.8 ANGSTROM CRYSTAL STRUCTURE OF THE DIMERIC PEROXISOMAL THIOLASE OF SACCHAROMYCES CEREVISIAE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.170, 92.650, 116.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.187 |
| Rwork | 0.203 |
| R-free | 0.24200 * |
| Structure solution method | MIRAS |
| RMSD bond length | 0.017 |
| RMSD bond angle | 2.400 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | TNT |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.047 | 0.122 |
| Number of reflections | 71570 | |
| <I/σ(I)> | 24.8 | 12.4 |
| Completeness [%] | 98.9 | 86.8 |
| Redundancy | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 6.5 | 293 | MICRODIALYSIS AGAINST 25MM MOPS PH6.5, 200MM DTT, 1MM EDTA, 1MM SODIUM AZIDE, AT 20 DEGREES CELSIUS, microdiaylsis, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | MOPS | 25 (mM) | |
| 2 | 1 | 1 | DTT | 1 (mM) | |
| 3 | 1 | 1 | 1 (mM) | ||
| 4 | 1 | 1 | EDTA | 1 (mM) |
