1AFD
STRUCTURAL BASIS OF GALACTOSE RECOGNITION IN C-TYPE ANIMAL LECTINS
Experimental procedure
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-07-06 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.400, 84.700, 98.000 |
| Unit cell angles | 90.00, 105.40, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.234 |
| Rwork | 0.234 |
| R-free | 0.28200 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.54) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.000 | 2.000 * |
| Rmerge | 0.049 | 0.214 * |
| Number of reflections | 41049 * | |
| Completeness [%] | 92.9 | 92.9 * |
| Redundancy | 3.2 | 2.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 | 20 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | 10 (mM) | ||
| 4 | 1 | drop | MPD | 20 (%) | |
| 5 | 1 | reservoir | PEG8000 | 12-15 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 7 | 1 | reservoir | 20 (mM) | ||
| 8 | 1 | reservoir | 0.02 (%) |
