1AF2

CRYSTAL STRUCTURE OF CYTIDINE DEAMINASE COMPLEXED WITH URIDINE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	297
Detector technology	IMAGE PLATE
Collection date	1993-02
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 31 2 1
Unit cell lengths	120.300, 120.300, 78.400
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	7.000 - 2.300
R-factor	0.19 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ctu
RMSD bond length	0.009 *
RMSD bond angle	2.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.0)
Refinement software	X-PLOR (3.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.500
High resolution limit [Å]	2.300	2.300
Rmerge	0.075	0.200
Total number of observations	65003 *
Number of reflections	24137
<I/σ(I)>	12	2.5
Completeness [%]	80.0	70
Redundancy	2.7	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2	4 *	pH 6.2

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	14-20 (%sat)
2	1	drop	protein	11-16 (mg/ml)
3	1	drop	uridine	20 (mM)
4	1	reservoir	ammonium sulfate	32-45 (%sat)