1AEW

L-CHAIN HORSE APOFERRITIN

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.5
Synchrotron site	SRS
Beamline	PX9.5
Temperature [K]	290
Detector technology	IMAGE PLATE
Collection date	1993-02
Detector	MARRESEARCH
Spacegroup name	F 4 3 2
Unit cell lengths	184.000, 184.000, 184.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.950
R-factor	0.192
Rwork	0.192
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	HORSE SPLEEN L FERRITIN.
RMSD bond length	0.008
RMSD bond angle	14.940 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((AGROVATA)
Refinement software	TNT (5D)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.030	0.120
Number of reflections	19915
<I/σ(I)>	17.6	6.3
Completeness [%]	100.0	97.5
Redundancy	7.6	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7		CRYSTALLIZED BY SITTING DROP: 15 LAMBDA PROTEIN + 1-2 LAMBDA 2% CDSO4, pH 7.0, vapor diffusion - sitting drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)	0.015 ml
2	1	drop		2 (%)	0.001-0.002 ml