1ADY
HISTIDYL-TRNA SYNTHETASE IN COMPLEX WITH HISTIDYL-ADENYLATE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-01-31 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 171.300, 214.700, 49.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.800 |
| R-factor | 0.26 |
| Rwork | 0.260 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | HISTIDYL-TRNA SYNTHETASE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.184 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | |
| High resolution limit [Å] | 2.800 | |
| Rmerge | 0.100 | |
| Total number of observations | 85481 * | |
| Number of reflections | 32840 | |
| <I/σ(I)> | 5 | |
| Completeness [%] | 65.0 | 30 * |
| Redundancy | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | histidyl-tRNA | 6 (mg/ml) | |
| 2 | 1 | drop | tRNAHis | 4.3 (mg/ml) | |
| 3 | 1 | drop | 3-10 (mM) | ||
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | ammonium sulphate | 15 (%(v/v)) | |
| 6 | 1 | drop | bis-tris propane | 100 (mM) | |
| 7 | 1 | reservoir | ammonium sulphate | 50 (%sat) |
