1ADU

EARLY E2A DNA-BINDING PROTEIN

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	293
Detector technology	IMAGE PLATE
Collection date	1994-06-27
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	61.000, 91.200, 149.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.000
R-factor	0.201
Rwork	0.201
R-free	0.29600
RMSD bond length	0.006
RMSD bond angle	23.600 *
Data reduction software	XDS
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.000	3.000 *
Rmerge	0.168	0.496 *
Total number of observations	73641 *
Number of reflections	17172
Completeness [%]	99.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	0.5 (%)
2	1	reservoir	ammonium sulfate	13 (%sat)