1ADJ
HISTIDYL-TRNA SYNTHETASE IN COMPLEX WITH HISTIDINE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID2 |
| Synchrotron site | ESRF |
| Beamline | ID2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-09-24 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 171.300, 214.700, 49.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.27700 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.497 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.050 | 0.200 |
| Total number of observations | 214739 * | |
| Number of reflections | 49630 | |
| <I/σ(I)> | 10.5 | 3.4 |
| Completeness [%] | 99.0 | 97 |
| Redundancy | 4.3 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | histidyl-tRNA | 6 (mg/ml) | |
| 2 | 1 | drop | tRNAHis | 4.3 (mg/ml) | |
| 3 | 1 | drop | 3-10 (mM) | ||
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | ammonium sulphate | 15 (%(w/v)) | |
| 6 | 1 | drop | bis-tris propane | 100 (mM) | |
| 7 | 1 | reservoir | ammonium sulphate | 50 (%sat) |
