1AD2
RIBOSOMAL PROTEIN L1 MUTANT WITH SERINE 179 REPLACED BY CYSTEINE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.100, 61.400, 46.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.900 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | NAT L1 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 25.140 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.059 | 0.320 |
| Total number of observations | 116835 * | |
| Number of reflections | 24181 | |
| <I/σ(I)> | 8.5 | |
| Completeness [%] | 98.8 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 10 * | Nikonov, S., (1996) EMBO J., 15, 1350. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12-15 (mg/ml) | |
| 2 | 1 | drop | glycine-NaOH | 50 (mM) | |
| 3 | 1 | drop | methane pentanediol | 5 (%(v/v)) | |
| 4 | 1 | drop | ammonium sulfate | 30 (%sat) | |
| 5 | 1 | reservoir | ammonium sulfate | 60 (%sat) | |
| 6 | 1 | reservoir | methane pentanediol | 7 (%) |
