1ACD

V32D/F57H MUTANT OF MURINE ADIPOCYTE LIPID BINDING PROTEIN

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	AREA DETECTOR
Collection date	1994-04-22
Detector	SIEMENS
Spacegroup name	C 2 2 21
Unit cell lengths	79.490, 97.290, 50.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.700
R-factor	0.198
Rwork	0.198
R-free	0.27600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lib
RMSD bond length	0.010
RMSD bond angle	26.700 *
Data reduction software	XENGEN
Data scaling software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.860
High resolution limit [Å]	2.700	2.700
Rmerge	0.131 *
Total number of observations	54873 *
Number of reflections	26696
<I/σ(I)>	10.4	0.99
Completeness [%]	97.2	57.3
Redundancy	5	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5 *		2.4M PHOSPHATE, PH 6.4

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	12.5 (mM)
2	1	reservoir	protein	10 (mg/ml)
3	1	reservoir	phosphate	2.4 (M)