1ABW
DEOXY RHB1.1 (RECOMBINANT HEMOGLOBIN)
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 295 |
| Detector technology | AREA DETECTOR |
| Collection date | 1993-03 |
| Detector | SIEMENS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.900, 81.980, 53.920 |
| Unit cell angles | 90.00, 98.99, 90.00 |
Refinement procedure
| Resolution | 5.000 - 2.000 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hhb |
| RMSD bond length | 0.014 |
| RMSD bond angle | 20.500 * |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.000 | 2.200 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.046 | |
| Total number of observations | 70840 * | |
| Number of reflections | 34054 | |
| Completeness [%] | 77.1 | 56.1 |
| Redundancy | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 * | pH 6.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | PEG3350 | 6.5 (%) | |
| 3 | 1 | drop | ammonium acetate | 75 (mM) | |
| 4 | 1 | drop | KCN | 0.25 (mM) | |
| 5 | 1 | reservoir | PEG3350 | 13 (%) | |
| 6 | 1 | reservoir | ammonium acetate | 150 (mM) | |
| 7 | 1 | reservoir | KCN | 10 (mM) |
